However, we used to treat advanced tumors patients with autogenous CIK cells but found that programmed death ligand 1 (PD-L1), lymphocyte activation gene-3 (LAG-3), T-cell immunoglobulin and mucin domain-3 (TIM-3), and carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM-1) were related to reduced anti-tumor activities during cultivation of CIK cells [28]. This evidence concerns the gene CD274 and neoplasm.