Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) are clinical markers for liver damages and diseases, and higher levels of serum ALT and AST indicate liver dysfunction.20 As Figure 6A,B shown, HFD feeding induced obviously higher levels of ALT and AST (P < 0.01), but treatment with miR‐33a ASO could significantly attenuate these disorders. This evidence concerns the gene GPT and Decreased liver function.