2012). In diabetic rats renal Mmp2 expression was increased, and aldosterone antagonists showed the most robust effect in minimizing Mmp2 expression. Expression of the tissue inhibitor of matrix metalloproteinase 1 (Timp1) also increased in diabetes, following the increase in Mmp2. This change was also alleviated by losartan and eplerenone treatment (Fig. 3B). The expression of Timp2 and Mmp9 remained the same in all groups (data not shown). Here, TIMP2 is linked to diabetes mellitus.