In order to address whether the increased metastatic colonization in KO hosts could also be facilitated by an increase in the direct adhesion of melanoma cells to KO endothelial cells, CD102+ lung endothelial cells (LEC) were isolated by two rounds of MACS from single cell suspensions from naïve WT or KO lungs, resulting in >85% CD31-positive cells which readily formed vascular tubes in vitro (Supplementary Figure 2); lung fibroblasts (LF) were isolated as the CD102- flow-through adherent cell fraction (Figure 4A), incapable of vascular tube formation (Supplementary Figure 2). The gene discussed is PECAM1; the disease is melanoma.