For HuCCT1 bile duct cancer cells in 3-D culture, aML5-PEG11 induced marginal activation/phosphorylation of the MET receptor, AKT, and ERK (5%, 30%, and 35% activities of HGF at 10 minutes after stimulation) did not influence gene expression profiles responsible for bile duct development and was unable to induce tubulogenesis. The gene discussed is HGF; the disease is bile duct cancer.