Cell-Collector is based on a sterile stainless steel medical wire, covered with 2 μm gold and a hydrogel layer which is covalently coupled with antibodies against the EpCAM protein and pan-keratins. CD45 staining (performed to exclude unspecific leucocytes) CTCs +: CTCs identified as pan-keratin positive, leukocyte marker CD45 negative. EPISPOT on an EpCAM-independent enrichment method (i.e., leukocyte depletion) and enables the identification of viable PSA-secreting tumor cells CTCs+: PSA+ cells. Here, EPCAM is linked to neoplasm.