By our analysis, ZBTB33 is more likely to bind to the string with minor allele G of rs157580 (Figure 5), compared with the string containing major allele A. The MAF of rs157580 in AD group was less than that in the CN group (Table 3), suggesting that the regulatory function of ZBTB33 in AD patients is weaker than that in the healthy people. Here, ZBTB33 is linked to Alzheimer disease.