In this work, by evaluating the innate antiviral immune response triggered by TLR3 activation and rotavirus infection in PIE cells we demonstrated that L. delbrueckii TUA4408L and its EPS are able to modulate IRF3 and NF-κB signaling pathways, improve IFN-β, MxA, and RNaseL expression, and significantly reduce rotavirus replication (Figure 8B). Here, IRF3 is linked to Rotavirus infection.