PFN1 and skin basal cell carcinoma: Although, FLP defect has been causally linked to reduced FAK/ERK activation in BCC, surprisingly, immunoblot analyses of 3D BME-MoT culture extracts showed no discernible evidence of impairment in either the total protein expression or activation (as judged by the levels of phosphorylation) of FAK and ERK in MDA-231 cells, at least at a global level, upon transient KD of either Pfn1 or Pfn2 (Fig. 3c).