The inverse correlation between SMAD3 and the outgrowth phenotype of Pfn1 KD MDA-231 cells in BME vs collagen-I-supplemented BME cultures prompted us to further probe whether could be a potential causal link between SMAD3 hyperactivation and outgrowth deficiency of BCC upon LOF of Pfn1. This evidence concerns the gene PFN1 and skin basal cell carcinoma.