Our data indicate a role for both ZF1 and ZF2 of EVI1 in myeloid skewing and in the upregulation of key myeloid regulator, Spi1. To further examine the role of EVI1 in transcriptional regulation of Spi1, we performed Chromatin Immunoprecipitation Sequencing (ChIP-Seq) on DA.1 cells, an EVI1-overexpressing AML. This evidence concerns the gene MECOM and acute myeloid leukemia.