FN1 and esophageal squamous cell carcinoma: To assess the motility and migration ability of ESCC cells in a setting of high stromal FN content, we cultured the ESCC cell lines, Eca-109 and TE-1, in the presence of MSC-conditioned medium (experimental group), mimicking a high stromal FN microenvironment, or DMEM (low-sugar) medium (control group).