The selection of mutational targets for ctDNA analysis was based on the following criteria: (a) known melanoma hotspot mutation in BRAF, NRAS, and/or TERT promoter; (b) COSMIC/TCGA reported mutation; (c) other mutation with a polyPhen score >0.7 and high variant allele frequency (VAF) in the tumor. The gene discussed is BRAF; the disease is melanoma.