Furthermore, the prognostic value of CLCA4 in different risk of subgroups according to tumor size, vascular invasion, TNM stage and BCLC stage was also analyzed, which found that CLCA4 could maintain its prognostic value in different risk of HCC patients for except OS and TTR in patients who had vascular invasion or OS in patients with tumor size ≤ 5 cm. This evidence concerns the gene CLCA4 and hepatocellular carcinoma.