To test whether overexpression of TRIM28 affects the exogenous expression of MAGEC2, FLAG-tagged MAGEC2 or FLAG-tagged MAGEA3 and pCL-TRIM28 expression plasmids were co-transfected into human pancreatic cancer cell line AsPC1, which has no expression of MAGEC2 and much lower level of TRIM28 expression compared with that in A375 and Hs 695 T cells (Additional file 1: Figure S1). This evidence concerns the gene TRIM28 and pancreatic neoplasm.