It has been previously reported that six–eight weeks of B. longum 35624 supplementation increases secretion of Treg cells in the peripheral blood of healthy human subjects as well as reduce systemic circulation of pro-inflammatory biomarkers TNF-α and IL-6 in clinical patients diagnosed with chronic fatigue syndrome (CFS) an immune-inflammatory disease [9,12]. This evidence concerns the gene IL6 and myalgic encephalomeyelitis/chronic fatigue syndrome.