It has been previously reported that six–eight weeks of B. longum 35624 supplementation increases secretion of Treg cells in the peripheral blood of healthy human subjects as well as reduce systemic circulation of pro-inflammatory biomarkers TNF-α and IL-6 in clinical patients diagnosed with chronic fatigue syndrome (CFS) an immune-inflammatory disease [9,12]. Here, TNF is linked to myalgic encephalomeyelitis/chronic fatigue syndrome.