Next, to test our hypothesis that acetylation of AGO2 promotes tumorigenesis by specifically increasing mature miR-19b, we used a lung carcinoma cell line A549 to generate stable cell lines expressing the control vector, pre-miR-19b1, AGO2-WT, AGO2-WT-pre-miR-19b1, AGO2-3KR and AGO2-3KR-pre-miR-19b1. The gene discussed is AGO2; the disease is lung carcinoma.