Finally, we also generated MD domain deletion constructs and found that the deletion of MD fragment within Hsp90ab1 abolished the ability of Hsp90ab1 to interact with LRP5 by GST pulldown assays and exhibited a weak effect on promoting tumor aggressiveness compared with the overexpression group in vitro experiments (Fig. 6i, Figure S5). The gene discussed is HSP90AB1; the disease is neoplasm.