RBE, a BAP1high ICC cell line, was transfected with lentiviral vector encoding short hairpin BAP1 (shBAP1) to establish an ICC cell line with stable downregulation of BAP1 (RBE-shBAP1) and compared with its control (RBE-Mock) (Fig. 2a). The gene discussed is BAP1; the disease is intrahepatic cholangiocarcinoma.