PLAGL2 and colorectal carcinoma: To further determine the functional role of PLAGL2 in miR-486-5p-induced inhibition of proliferation and migration in CRC, the expression of PLAGL2 was knocked down using siPLAGL2 and WB assay confirmed that siPLAGL2 specifically decreased the PLAGL2 protein expression level in HT29 and SW480 cells (Fig. 5a).