CPN1 and infection: The wild-type untransformed C. pneumoniae LPCoLN inclusions have no naturally occurring fluorescence; in contrast, a strong red-shifted green fluorescent protein (RSGFP) signal was detected in C. pneumoniae LPCoLN (C. pneumoniae LPCoLN-pRSGFPCAT-Cpn) inclusions after transformation of this recipient with the recombinant shuttle vector and infection in HEp-2 cells (Fig. 1B).