ARHGEF16 and central nervous system cancer: In contrast to the increased migration and proliferation capacities of ARHGEF16-overexpressing U87 or U118 cells, ARHGEF16 knockdown in H4 cell (Fig. 4a, b) greatly inhibited glioma cell proliferationas assessed via plate colony formation (Fig. 4c, d) and EdU (Fig. 4e, f) assays.