A subsequent semiquantitative RT-PCR analysis using primers for AZIN2 mRNA detected the expression of AZIN2 in different regions of the rat brain (frontal cortex, hippocampus, hypothalamus, locus coeruleus, medulla and striatum) and the human brain (frontal cortex, hippocampus and nucleus accumbens), and in cultured rat neuronal cells (neurons, PC12, astrocytes and glioma cells) [100]. The gene discussed is AZIN2; the disease is central nervous system cancer.