POU5F1 and cancer: Earlier germ cell-like cells, as determined by co-expression of Oct4 and DAZL as well as expression of Ifitm3, could be detected both in wild-type (RKO, U2OS, LNCaP, and HCT116) and p53-null (Hep3B, PC3, and p53−/− HCT116) cancer cell lines (Fig. 4a, b).