To investigate the role of LEM4 in breast cancer, we performed immunohistochemistry (IHC) with commercial tissue microarrays (HBre-Duc150-Sur-01/02) and found that LEM4 was more highly expressed in tumor tissues from breast cancer patients and weakly detected in the paired noncancerous tissue regions (Fig. 1b, c). This evidence concerns the gene ANKLE2 and neoplasm.