CD4+ T cell help was not necessary for this enhancement, suggesting that the increased CD8+ T cell response is due to a direct effect on transduced DC, leading to more efficient presentation of antigen peptides on MHC class I. In this study, we analysed TRAP expression in transfected A549 cells (human adenocarcinoma epithelial cell line) to determine whether Ii has an impact on intracellular trafficking of the antigen. The gene discussed is CD4; the disease is adenocarcinoma.