The results indicated that apelin was increased to 4.3 folds in glomeruli of type 2 diabetic mice (kkAy mice) as shown in Fig. 1a, b, apelin mRNA was increased to 7.1 folds in glomeruli of kkAy mice as shown in Fig. 1c, apelin concentration in homogenate was increased from 255 pg/μg to 362 pg/μg in glomeruli of kkAy mice as shown in Fig. 1d. This evidence concerns the gene APLN and type 2 diabetes mellitus.