The main findings of the present study are: (I) Uremia impacts the cellular adhesion and disturbs the homeostasis of endothelial cell phenotype; (II) Uremic environment modifies the endothelial cell cytoskeleton and F-actin protein expression; (III) Uremic environment impacts endothelial cell adherent junction and VE-cadherin expression, and (IV) Uremic environment compromises tight junction and differently modulates ZO-1 gene and protein expression. Here, CDH5 is linked to uremia.