CASP3 and cancer: Moreover, the treatment of HCT116 cells and LS174 cells with 8 μM of (+)-UA for 24 or 48 hours did not remarkably induce the increase in CASP3/7 activity (Figures 4(e) and 4(f)), which suggested that treatment of some cancer cell lines with low dose of (+)-UA is not yet sufficient to increase the permeability of mitochondrial membrane [19].