HIF1A and ischemia: Because the restoration of blood supply often causes more damage to the tissue than the ischemic episode itself, we analyzed the effect of reperfusion at different time intervals (Figure 2C): compared with control, the level of HIF-1α expression increased rapidly after ischemia (∗∗∗P < 0.001); however, no significant differences were observed between 0 and 24 h of reperfusion.