NOTCH1 and medulloblastoma: We transfected Group 3 medulloblastoma cells (D425 and D283) with two constructs: 1) EF1.GFP.T2A.Luc, for constitutive expression of GFP and luciferase and 2) doxycycline/RFP-inducible short hairpin RNA (shRNA) to NOTCH1, for reducing NOTCH1 expression (Supplementary Fig. 6 and Supplementary Table 2).