Since (1) NOTCH intracellular domain directly binds to the TWIST1 promoter23,24, (2) BMI1 is a direct transcriptional target of TWIST125, and (3) BMI1 has been shown as a NOTCH126 and TWIST125 downstream target, we hypothesized that the TWIST1–BMI1 axis24,27 is involved in NOTCH1-induced Group 3 medulloblastoma metastasis. The gene discussed is TWIST1; the disease is medulloblastoma.