The gene panel used was designed for genomic analysis of gastrointestinal tumours and comprised amplicons of relevant DNA regions from 12 genes including BRAF, DDR2, ERBB2, HRAS, KEAP1,KRAS, NFE2L2,NRAS,PIK3CA, PTEN, RHOA and TP53 (see Supplemental Table S2). The gene discussed is BRAF; the disease is digestive system neoplasm.