We examined the impact of ALS-associated mutations on cholinergic motor neuron survival in single-copy and overexpression models for ALS SOD1 in C. elegans. Survival of cholinergic motor neurons was assessed based on retention/loss of GFP or mCherry in animals carrying the unc-17p::GFP or cho-1p::mCherry transgenes, respectively, which express fluorescent proteins specifically in cholinergic neurons (Fig 3A). This evidence concerns the gene SOD1 and amyotrophic lateral sclerosis.