In the seminoma cell line TCam-2, the PIWI-LIKE 2 promoter is heavily methylated (85%), whereas in the pluripotent embryonal carcinoma cell line NT2/D1, the PIWI-LIKE 2 promoter exhibits a low promoter methylation (22%) (Figure 1B). The gene discussed is PIWIL2; the disease is embryonal carcinoma.