Relapse specific mutations were investigated and identified in four patients with required resistance to PD-1 blockade therapy in melanoma, including loss of function of JAK1, JAK2, and B2M, which induces either lack of response to interferon gamma (IFNγ), or loss of surface expression of major histocompatibility complex I (MHC I) (Zaretsky et al., 2016). Here, B2M is linked to melanoma.