To further confirm that CLANmCas9/gNLRP3 improved the symptoms of HFD-induced T2D through NLRP3 knockout-mediated inhibition of the NLRP3 inflammasome, we detected the expression of IL-1β, cleaved caspase-1 p10 and NLRP3 in white adipose tissue (WAT) of mice with different treatments. This evidence concerns the gene CASP1 and type 2 diabetes mellitus.