To investigate the potential molecular mechanisms underlying impaired neural tube closure in SR-B1 KO embryos, we performed an unbiased gene expression analysis by massive mRNA sequencing in E9.5 WT embryos and SR-B1 KO embryo with the two distinct phenotypes: KO-N and KO-NTD, retrieved from SR-B1 heterozygous intercrosses. The gene discussed is SCARB1; the disease is neural tube defect.