We used transcriptional profiling by RNA-Seq in wild-type (WT), SR-B1 KO morphologically normal embryos (KO-N) and SR-B1 KO embryos with NTD (KO-NTD) to isolate specific genes and assign potential biological processes that may be altered in KO-NTD embryos. The gene discussed is SCARB1; the disease is neural tube defect.