TSPAN8 and colon adenocarcinoma: To determine the expression of cell adhesion molecules, we performed immunoblotting and qRT-PCR analysis for claudin-1, −3, −4 and −7, EpCAM, E-cadherin, vimentin, Tspan8, CD9 and CD82 in normal human colon cells CCD18Co, colon adenocarcinoma cell lines Caco-2 and DLD-1, and metastatic colon cancer cell line SW620.