To examine which caspase activation is important for the induction of M. smegmatis-mediated apoptosis in macrophages, RAW 264.7 cells were pretreated with z-ATAD-fmk (caspase-12 inhibitor), z-LEHD-fmk (caspase-9 inhibitor), and z-VAD-fmk (pan-caspase inhibitor) following infection with M. smegmatis, and caspase activation was evaluated. This evidence concerns the gene CASP9 and infection.