However, this CCCP/iron-induced pyroptosis seemed specific to melanoma cells since other types of cell lines, Huh7 hepatoma cells that did not express GSDME was resistant to CCCP/iron stimulation, whereas A549 lung cancer cells and MDA-MB-231 breast cancer cells that expressed a comparable level of GSDME with A375 melanoma cells, were not as sensitive as A375 cells to CCCP/iron-induced pyroptosis (Supplementary information, Fig. S1n). This evidence concerns the gene GSDME and hepatocellular carcinoma.