Consistent with the literature27,28, treating LNCaP cells with MDV3100 or culturing another AR-positive prostate cancer cell line 22Rv1 in CSS medium has resulted in induction of H3K27me3 and NE markers (Fig. 2a), which suggests that ADT activates EZH2’s PRC2 activity. Here, EZH2 is linked to Familial prostate cancer.