These results indicated that HOXC-AS3 may participate in the tumorigenesis of GC through the transcriptional regulation of other genes via binding to YBX1 in trans. To probe the HOXC-AS3-associated pathway on an unbiased basis in the tumorigenesis of GC, RNA-Seq assays were used after simultaneous knockdown HOXC-AS3 and YBX1. This evidence concerns the gene YBX1 and gastric cancer.