The growth curves indicated that the effect of RHBDD1 deletion differed modestly between the two cell lines, and a significant decrease in growth rate was observed in MDA-MB-231 knock-out cells compared with wild-type cells, although RHBDD1 levels exhibited no significant association with triple-negative breast cancer (TNBC) in clinical specimens. The gene discussed is RHBDD1; the disease is triple-negative breast carcinoma.