Analyzing the effect of diet and CrM at the mTOR protein level, we observed that there was no difference in total mTOR normalized to GAPDH among the treatments (Fig 6F); however, HF-T treatment significantly reduced the normalized protein levels of phosphorylated mTOR in comparison to SD-T and in HF-T-CrM in comparison to SD-T-CrM (Fig 6G). Here, MTOR is linked to hydrops fetalis.