The hypomethylation status of the ZBTB7A CpG326 located at the gene’s proximal promoter region could lead to a possible elevated expression of LRF (leukemia/lymphoma-related factor) and the protein product of ZBTB7A gene and could have emerged the failure of γ-globin induction above the limit of the 2.1-fold (maximum observed γ-globin relative expression), on account of the erythropoietic cells’ exposure to the HU agent among non-responders. This evidence concerns the gene ZBTB7A and leukemia.