In current study, the infiltration of four immune cell types (CD8+ /CD4+/Foxp3+/CD33+ cells), the expression of both inhibitory (PD-1/PD-L1/Tim-3/LAG-3) and stimulatory checkpoints (OX-40/ICOS), and IDO1 were evaluated in two independent cohorts with ESCC (95 patients in the primary cohort and 55 patients in the validation cohort). The gene discussed is FOXP3; the disease is esophageal squamous cell carcinoma.