Second, EMT was induced in the human breast cancer cell line MCF7 by downregulation of E-cadherin using stable expression of shRNA [23] and, thirdly, EMT was induced in normal murine mammary epithelial (NMuMG) cells by treatment with TGFβ [54] (Additional file 1: Figure S1A). This evidence concerns the gene CDH1 and breast carcinoma.