It is well‐established that, at 40 weeks, O‐T2D are hypoinsulinemic due to β‐cell insufficiency,40, 41, 42, 43 whereas O‐CON had reduced insulin levels due to energy restriction.(28) In the subset of animals that underwent the hyperinsulinemic, euglycemic clamp, O‐T2D had reduced whole‐body insulin sensitivity compared to O‐CON, as shown by the lower glucose infusion rate (GIR) required to maintain euglycemia (O‐T2D = 9 ± 3 mg · kg−1 · min−1; O‐CON = 23 ± 3 mg · kg−1 · min−1; p = 0.001). This evidence concerns the gene INS and type 2 diabetes mellitus.