TIB supernatant samples in serum-free medium (no human serum or FBS) were incubated in three 12-plex peptide microarray chips [format described in (48), which was previously reported from our group for human serum IgG reactivity to the H1N1 influenza proteome] embedded with 12-mer peptides from the human KRAS protein (UniProt ID: P01116) covering the most frequently occurring point mutations associated with cancer: G12D, G12V, G12R, G12S, G12C, G13D, Q61K, Q61H, Q61R, and A146T. The gene discussed is KRAS; the disease is cancer.