Furthermore, to distinguish early apoptotic cells from late apoptotic or necrotic cells after overexpression of BAD gene in GCs, Annexin V fluorescein isothiocyanate/propidium iodide (FITC/PI) flow cytometric analysis was performed using our BAD-overexpressing cell line at days 5, 7, 11, and 13 after infection. The gene discussed is ANXA5; the disease is infection.