Analysis of VEGFR1, VEGFR2, FGFR1, FGFR3 and VE-Cadherin expression levels in B16F0 melanoma cells, and tumors isolated from control and DCKO mice showed ~30% reduced levels of VEGFR2 (but not VEGFR1) in DCKO mice compared to DFF control littermates, consistent with Vegfr2 haploinsufficiency (Supplementary Fig. S2A,B). This evidence concerns the gene KDR and melanoma.